dna ligase meaning in English
dna连接酶,多脱氧核糖核苷酸合酶
dna黏合酶
脱氧核糖核酸连接酶
Examples
- Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants
将cdna与载体连接，并导入dh5感受态细胞中，构建成cdna文库。 - 4 . the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi , and collected the digested cpti fragment and the pgem - 7z , then ligated by t4 dna ligase and formed the pgem - cp
中间载体及表达载体的构建将puc - cp质粒和pgem ? 7z质粒，用kpni和bamhi酶切，分别回收cpti片断和酶切后的载体片段，用t _ 4连接酶连接构建成中间载体pgem - cp 。 - Sub - - clone of s , . / hbsag fusion gene : pbuescripts , . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme , and were linked under t4 dna ligase , ppiczaa s , , / hbsag was constructed and transformed to e . coli
Hbsag质粒与ppiczaa载体分别经xhol和xbaln切，再在t4dna连接酶作用下进行连接，获得工程菌表达型ppiczaas ； hbsag质粒，转化大肠杆菌t0p10细胞，经xhol和xbal与sacll和xbal酶切电泳，证实s ； 。 - At first . then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr . following that , these gene fragments and plasmid vectors , pbluescript ii ks + and puc18 , were cut by bamh i and kpn i . the prepared insert dna and vector dna were linked by t4 dna ligase
利用vectornti6 . 0软件，对所克隆的序列用相邻接点法( neighborjoining州j ） method ）进行多序列比对，分析其同源性，并构建基因进化树。 - The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme , were linked by means of t4 dna ligase and transformed into e . coli jm109 permissive cells , yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
将重组质粒pugedna与转移载体pfastbacldna用bamhi和ecori双酶切处理， t _ 4dna连接酶连接，用连接产物转化大肠杆菌jm109感受态细胞，得到重组转移载体质粒pfastbac - gedna 。